g3 treated with e coli o55 b5 atcc 12014 positive control  (ATCC)

Journal: Veterinary World

Article Title: Farm-adapted multicomponent bacteriophage preparation for environmental biocontrol in commercial dairy cattle housing: A One Health approach

doi: 10.14202/vetworld.2026.3883-3898

Figure Lengend Snippet: Host range and efficiency of plating of Escherichia phage vB_Eco_BCPEco_001.

Article Snippet: Reference , ATCC 12014 , 4+ (CL) , 1 , − , −.

Techniques:

Journal: Extracellular vesicle

Article Title: Exosomal delivery of IL-10: Biodistribution, pharmacokinetics, and preterm birth prevention strategies

doi: 10.1016/j.vesic.2025.100066

Figure Lengend Snippet: (A) Schematic representation of the ascending infection-induced preterm birth mouse model and intravenous treatment with eIL-10 (500 ng). Infection is induced by vaginal administration of E. coli (10^10 colony-forming units [CFU]). (B) Survival graph comparing PBS (black line), E. coli (dashed black lines), E. coli + Naïve EVs (green), E. coli + tIL-10 (red), E. coli + eIL-10 (blue) and E. coli + rIL-10 (grey) treatment groups. E. coli treatment caused PTB within 48 h, while E. coli + tIL-10 and E. coli + eIL-10 delayed PTB to term delivery with live pups, whereas E. coli + rIL-10 treatment delays only 24 h compared to the E. coli group. For all groups, n ≥ 4. (C) The Delay in preterm birth (in hours) is shown for each treatment group. The color key is as in . (D) Live pups from tIL-10 and eIL-10 were weighed, and the ratio of the number of pups to their weights was shown in bar graphs. Data are shown as means ± SEM. Data were analyzed using a one-way ANOVA with Tukey’s post hoc test. *P ≤ 0.05, **P ≤ 0.05 ***P ≤ 0.001. “ns” represents not significant.

Article Snippet: Briefly, on gestational day E–15, CD-1 mice were vaginally administered with 10 10 CFU E. coli ( ATCC 12014 Escherichia coli O55:K59(B5): H., Thermo Fisher Scientific, Remel Products, Lenexa, KS, USA, Lot# 496291).

Techniques: Infection

Journal: Microbiology Spectrum

Article Title: Integrated and high-throughput method to collect, store, recover, and manage microbial isolates in mini-arrays

doi: 10.1128/spectrum.02637-24

Figure Lengend Snippet: Recovery of microbes after 6 months ^a

Article Snippet: E. coli strain ATCC12014 , S. aureus strains ATCC49525 and ATCC 6538, Shigella sonnei strain ATCC 9290, and the Shigella dysenteriae strain ATCC 9750 were obtained from ATCC (Manassas, Virginia).

Techniques:

Journal: Microbiology Spectrum

Article Title: Integrated and high-throughput method to collect, store, recover, and manage microbial isolates in mini-arrays

doi: 10.1128/spectrum.02637-24

Figure Lengend Snippet: Reliability of recovering microbes after 1 week

Article Snippet: E. coli strain ATCC12014 , S. aureus strains ATCC49525 and ATCC 6538, Shigella sonnei strain ATCC 9290, and the Shigella dysenteriae strain ATCC 9750 were obtained from ATCC (Manassas, Virginia).

Techniques:

Journal: Frontiers in Cell and Developmental Biology

Article Title: Characterization of fetal microchimeric immune cells in mouse maternal hearts during physiologic and pathologic pregnancies

doi: 10.3389/fcell.2023.1256945

Figure Lengend Snippet: Methodology framework for high-dimensional mass cytometry. (A) Male homozygous mT/mG +/+ C57BL/6J mice were mated with wild-type females, which resulted in progeny cells that express mT+. Pregnant mice were treated intravaginally with 10 4 CFU E. coli to mimic ascending infection, with sterile nutrient broth serving as negative control. (B) Both flow cytometry and mass cytometry utilize cell labeling with antibodies to facilitate single-cell analysis, although fewer spectral overlap is observed with mass cytometry. (C) Molecules of interest were tagged with elemental isotope-labelled antibodies. Following nebulization and injection into a mass analyzer, the ionized metals were accelerated through a vacuum chamber and were analyzed based on time of flight.

Article Snippet: A day prior to experiment, a bacterial culture of ATCC 12014 Escherichia coli O55:K59(B5):H– (Lot #496291, ThermoFisher Scientific Remel Products, San Diego, CA, United States) was inoculated in 200 mL sterile nutrient broth (Difco, Cat. # 234000, BD Biosciences, Franklin Lakes, NJ, United States) and cultured for 16 h at 37°C with shaking.

Techniques: Mass Cytometry, Infection, Sterility, Negative Control, Flow Cytometry, Labeling, Single-cell Analysis, Injection